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Applications and services of the Proteomics Core Facility Umeå

Through their collaboration, the Mass Spectrometry Proteomics Laboratory at the BMC Uppsala and the KBC Proteomics core Facility Umeå can now offer the following analytical applications:

  • Qualitative proteomics for identification of proteins in complex biological samples.
  • Proteogenomics for finding new gene models.
  • Quantitative proteomics using label-free and labeling techniques.
  • Body fluid proteomics, e.g., cerebrospinal fluid and plasma.
  • Analysis of proteins from tissue samples stored in biobanks (FFPE/OCT storage).
  • Proteomics of samples from non-human species, e.g., cats, dogs, plants, bacteria and parasites.
  • Characterization of post-translational modifications, e.g., protein phosphorylation and glycosylation.
  • Immunoprecipitation and identification of target proteins. The facility offers advice on experimental set up for identification of immunoprecipitated and co-immunoprecipitated proteins with MS.
  • Protein identifications from solution and gel spots/bands.
  • Protein extraction. Selective extraction of proteins from cells and tissues.
  • Determination of the intact mass of recombinant and native proteins.
  • In depth characterization of native and recombinant proteins.
  • Protein separation. 1-D SDS-PAGE, ion chromatography fractionation (SCX, ERLIC).
  • Data evaluation, documentation, and archiving.
  • Training services. We provide courses and workshops.

Please send us a brief description of your project including your scientific questions of interest and the type of samples to be analyzed. We will then get back to you to discuss a suitable experimental design to address those questions.

 

Contact

Thomas Kieselbach
Research coordinator
E-mail
Email