Cross-linking and Hydrogen-Deuterium Exchange Mass Spectrometry
Tue
30
May
Tuesday 30 May, 2023at 12:00 - 13:00
Glasburen, KBC, and Zoom
In the frame of the “SciLifeLab Site Umeå / KBC Infrastructure Seminar Series”, the seminars will be organised every second Tuesday during spring 2023. The seminars will be held in a hybrid format IRL in Glasburen, KBC, and online via Zoom. Learn more about the seminar series and Schedule for Spring 2023 here.
On May 30th, 12:00-13:00, SciLifeLab Site Umeå / KBC Infrastructure Seminar Series presents:
Cross-linking and Hydrogen-Deuterium Exchange Mass Spectrometry as a National Infrastructure Service
by
Simon Ekström
Swedish National Infrastructure for Biological Mass Spectrometry (BioMS), Lund University, Lund, Sweden and Structural Proteomics, SciLifeLab Integrated Structural Biology platform (ISB), Lund, Sweden
Sign up (latest by Monday, 29 May, 10 a.m.) for on-site attendance to reserve your free lunch sandwich:
(1) Division of Infection Medicine, Department of Clinical Sciences, Lund University, Lund, Sweden. (2) Swedish National Infrastructure for Biological Mass Spectrometry (BioMS), Lund University, Lund, Sweden. (3) Structural Proteomics, SciLifeLab Integrated Structural Biology platform (ISB), Lund, Sweden.
Cross-linking (XL-MS) and hydrogen-deuterium exchange mass spectrometry (HDX-MS) are powerful techniques in structural proteomics. XL-MS provides evidence of proteins interacting with each other as well as structural information of individual proteins or protein complexes by specifying (alt - identifying or providing?) distance constraints between two cross-linked peptides. The distance constraints arise from the defined length of the cross-linker used. HDX-MS analysis can be used to obtain information on structure, protein-protein interaction sites, allosteric effects, intrinsic disorder, and conformational changes. HDX-MS takes advantage of the labile nature of the exchangeable protons present on protein backbone amides. When dissolved in solution, proteins exchange these protons with hydrogen groups present in a deuterated buffer The rate of hydrogen to deuterium exchange can be measured by MS, and provides a measure of solvent accessibility and used to infer structural information. Examples from successful user projects will be shown, e.g large scale epitope mapping and XL-MS.
Organisers: SciLifeLab Umeå and Chemical Biological Centre (KBC)