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Umeå University Flow Cytometric Core Facility

Research infrastructure Flow cytometry enables simultaneously analysis of cell surface bound as well as intracellular proteins and RNA expression on a single cell basis. In addition, secreted proteins can be analyzed using a bead based systems.

Flow cytometry is a powerful tool for rapid analysis of single cells or particles. It allows for distinct phenotyping of cells based on their expression of surface- or intracellular molecules or proteins. The additional capacity for rapid cell sorting based on these characteristics also allows for downstream applications such as functional- or genetic/transcriptional analysis. The use of flow cytometry to measure fluorescently labelled beads in a multiplex setting also allows for simultaneous assessment of hundreds of biomarkers in one sample.

The Umeå University Flow Cytometric Core Facility provides state-of-the-art equipment and training for advanced analysis and cell sorting of single cells or detection of multiple biomarkers in solution. We can also aid with hands-on sample and equipment handling, if required.

Available equipment:

APPLICATION

EQUIPMENT

Phenotypic single cell analysis

5-laser Biorad ZE5 cell analyzer
2-laser BD Accuri cell analyzer

Bulk or single cell sorting

3-laser BD Melody (BSL-2 cell sorter)
4-laser BD Aria III cell sorter (BSL-2 sorting of non-infectious material)

Multiplex for biomarker analysis

BioRad Bioplex 200

ELISpot / Fluorospot

Mabtech IRIS 4 color Fluorospot

High capacity liquid handling

Hamilton Microlab Star automated pipetting robot

Platform manager (primary contact)

Kjell Eneslätt
Research engineer
E-mail
Email

Head of platform

Latest update: 2024-02-26