Lars Thelander

Making the building blocks to the genome

Research qualifications: Docent

Contact

Works at

Affiliated as professor emeritus at Department of Medical Biochemistry and Biophysics

KB. H6, Campus, Umeå Umeå universitet, 901 87 Umeå

The enzyme ribonucleotide reductase synthesize the building blocks to DNA. We want to understand how this synthesis is regulated in various organisms. We also study the nucleotide metabolism in Trypanosoms, a parasite that causes African sleeping sickness, with the goal to find new drug-targets.

De novo dNTP production is essential for normal postnatal murine heart development

Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology 2019, Vol. 394, (44) : 15889-15897

Tran, Phong; Wanrooij, Paulina H.; Lorenzon, Paolo; et al.

Trypanosoma brucei thymidine kinase is tandem protein consisting of two homologous parts, which together enable efficient substrate binding

Journal of Biological Chemistry, Vol. 287, (21) : 17628-17636

Ranjbarian, Farahnaz; Vodnala, Munender; Vodnala, Sharvani Munender; et al.

Metal binding and activity of ribonucleotide reductase protein R2 mutants: conditions for formation of the mixed manganese-iron cofactor

Biochemistry, Vol. 48, (27) : 6532-6539

Popović-Bijelić, Ana; Voevodskaya, Nina; Domkin, Vladimir; et al.

Ribonucleotide reduction is a cytosolic process in mammalian cells independently of DNA damage.

Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences 2008, Vol. 105, (46) : 17801-17806

Pontarin, Giovanna; Fijolek, Artur; Pizzo, Paola; et al.

Expression, purification, characterization, and in vivo targeting of trypanosome CTP synthetase for treatment of African sleeping sickness.

Journal of biological chemistry, Vol. 282, (16) : 11858-11865

Fijolek, Artur; Hofer, Anders; Thelander, Lars

p53R2-dependent ribonucleotide reduction provides deoxyribonucleotides in quiescent human fibroblasts in the absence of induced DNA damage

Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology 2007, Vol. 282, (23) : 16820-16828

Pontarin, Giovanna; Ferraro, Paola; Håkansson, Pelle; et al.

Ribonucleotide reductase and mitochondrial DNA synthesis.

Nature. Genetics, Vol. 39, (6) : 703-4

Thelander, Lars

The Schizosaccharomyces pombe replication inhibitor Spd1 regulates ribonucleotide reductase activity and dNTPs by binding to the large Cdc22 subunit.

Journal of Biological Chemistry, Vol. 281, (3) : 1778-1783

Håkansson, Pelle; Dahl, Lina; Chilkova, Olga; et al.

Regulation of mammalian ribonucleotide reduction and dNTP pools after DNA damage and in resting cells.

Journal of Biological Chemistry, Vol. 281, (12) : 7834-7841

Håkansson, Pelle; Hofer, Anders; Thelander, Lars

The involvement of Arg265 of mouse ribonucleotide reductase R2 protein in proton transfer and catalysis.

Journal of biological chemistry, Vol. 281, (36) : 26022-8

Narváez, Ana J; Voevodskaya, Nina; Thelander, Lars; et al.

Chlamydial ribonucleotide reductase: tyrosyl radical function in catalysis replaced by the FeIII-FeIV cluster.

Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, (26) : 9850-4

Voevodskaya, N; Narvaez, A-J; Domkin, Vladimir; et al.

S Phase-specific transcription of the mouse ribonucleotide reductase R2 gene requires both a proximal repressive E2F-binding site and an upstream promoter activating region.

Journal of biological chemistry, Vol. 279, (11) : 10796-807

Chabes, Anna Lena; Björklund, Stefan; Thelander, Lars

The ribonucleotide reductase R1 homolog of murine cytomegalovirus is not a functional enzyme subunit but is required for pathogenesis.

Journal of virology, Vol. 78, (8) : 4278-88

Lembo, David; Donalisio, Manuela; Hofer, Anders; et al.

Survival of DNA damage in yeast directly depends on increased dNTP levels allowed by relaxed feedback inhibition of ribonucleotide reductase.

Cell, Vol. 112, (3) : 391-401

Chabes, Andrei; Georgieva, Bilyana; Domkin, Vladimir; et al.

DNA building blocks at the foundation of better survival.

Cell Cycle, Vol. 2, (3) : 171-3

Chabes, Andrei; Thelander, Lars

Mouse ribonucleotide reductase R2 protein: A new target for anaphase-promoting complex-Cdh1-mediated proteolysis.

Proc. Natl. Acad. Sci. U S A., Vol. 100, (7) : 3925-3929

Chabes, Anna Lena; Pfleger, C.M.; Kirschner, M.W.; et al.

Sequences downstream of the transcription initiation site are important for proper initiation and regulation of mouse ribonucleotide reductase R2 gene transcription

European Journal of Biochemistry, Blackwell Publishing 2003, Vol. 270, (8) : 1791-1801

Kotova, Irina; Chabes, Anna Lena; Lobov, Sergei; et al.

Yeast DNA damage-inducible Rnr3 has a very low catalytic activity strongly stimulated after the formation of a cross-talking Rnr1/Rnr3 complex.

Journal of Biological Chemistry, Vol. 277, (21) : 18574-8

Domkin, Vladimir; Thelander, Lars; Chabes, Andrei

Cid13 is a cytoplasmic poly(A) polymerase that regulates ribonucleotide reductase mRNA.

Cell, Vol. 109, (5) : 563-73

Saitoh, Shigeaki; Chabes, Andrei; McDonald, W Hayes; et al.

Mammalian p53R2 protein forms an active ribonucleotide reductase in vitro with the R1 protein, which is expressed both in resting cells in response to DNA damage and in proliferating cells

Journal of Biological Chemistry, Vol. 276, (44) : 40647-40651

Guittet, Olivier; Håkansson, Pelle; Voevodskaya, Nina; et al.

Trypanosoma brucei CTP synthetase: a target for the treatment of African sleeping sickness.

Proceedings of the National Academy of Sciences of the U S A, Vol. 98, (11) : 6412-6

Hofer, Anders; Steverding, D; Chabes, Andrei; et al.

A mouse in vitro transcription system reconstituted from highly purified RNA polymerase II, TFIIH, and recombinant TBP, TFIIB, TFIIE and TFIIF.

European Journal of Biochemistry, Vol. 268, (16) : 4527-4536

Kotova, Irina; Chabes, Anna Lena; Segerman, Bo; et al.

Yeast ribonucleotide reductase has a heterodimeric iron-radical-containing subunit

Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, (6) : 2474-2479

Chabes, Andrei; Domkin, Vladimir; Larsson, Göran; et al.

Expression of an altered ribonucleotide reductase activity associated with the replication of murine cytomegalovirus in quiescent fibroblasts.

Journal of Virology, Vol. 74, (24) : 11557-65

Lembo, D; Gribaudo, G; Hofer, Anders; et al.

Mutational and structural analyses of the ribonucleotide reductase inhibitor Sml1 define its Rnr1 interaction domain whose inactivation allows suppression of mec1 and rad53 lethality.

Mol Cell Biol, Vol. 20, (23) : 9076-83

Zhao, X; Georgieva, B; Chabes, Andrei; et al.

Metabolic fate of oxidized guanine ribonucleotides in mammalian cells.

Biochemistry, Vol. 38, (12) : 3610-4

Hayakawa, H; Hofer, Anders; Thelander, Lars; et al.

Allosteric regulation of Trypanosoma brucei ribonucleotide reductase studied in vitro and in vivo.

Journal of Biological Chemistry, Vol. 273, (51) : 34098-104

Hofer, Anders; Ekanem, J T; Thelander, Lars

Two YY-1-binding proximal elements regulate the promoter strength of the TATA-less mouse ribonucleotide reductase R1 gene.

Journal of Biological Chemistry, Vol. 273, (45) : 29816-21

Johansson, Erik; Hjortsberg, K; Thelander, Lars

Cloning and characterization of the R1 and R2 subunits of ribonucleotide reductase from Trypanosoma brucei.

Proceedings of the National Academy of Sciences of the U S A, Vol. 94, (13) : 6959-64

Hofer, Anders; Schmidt, P P; Gräslund, Astrid; et al.

Induction of the mouse ribonucleotide reductase R1 and R2 genes in response to DNA damage by UV light.

Journal of Biological Chemistry, Vol. 271, (39) : 23698-704

Filatov, D; Björklund, Stefan; Johansson, Erik; et al.

The TATA-less promoter of mouse ribonucleotide reductase R1 gene contains a TFII-I binding initiator element essential for cell cycle-regulated transcription.

Journal of Biological Chemistry, Vol. 270, (50) : 30162-7

Johansson, Erik; Skogman, E; Thelander, Lars

Gene structure and regulation of the expression of the R1 and R2 subunits of mouse ribonucleotide reductase.

Advances in Experimental Medicine and Biology, Vol. 370 : 721-4

Johansson, Erik; Björklund, Stefan; Thelander, Lars

Structure and promoter characterization of the gene encoding the large subunit (R1 protein) of mouse ribonucleotide reductase.

Proceedings of the National Academy of Science U S A, Vol. 90, (23) : 11322-6

Björklund, Stefan; Hjortsberg, K; Johansson, Erik; et al.

An S-phase specific release from a transcriptional block regulates the expression of mouse ribonucleotide reductase R2 subunit

EMBO Journal, Vol. 11, (13) : 4953-4959

Björklund, Stefan; Skogman, E; Thelander, Lars

S-phase-specific expression of mammalian ribonucleotide reductase R1 and R2 subunit mRNAs

Biochemistry, Vol. 29, (23) : 5452-5458

Björklund, Stefan; Skog, Sven; Tribukait, Bernard; et al.

S phase-specific transcription of the mouse ribonucleotide reductase R2 gene is dependent on an upstream promoter activating region and a proximal repressive E2F binding site.

Chabes, Anna Lena; Björklund, Stefan; Thelander, Lars

Characterization of the promoters of the mouse ribonucleotide reductase R1, R2 and p53R2-genes

Elfving, Anna; Thelander, Lars

View publications in DiVA

Research projects

26 February 2007 until 31 December 2008

Regulation of deoxyribonucleotide synthesis during DNA replication and repair in eukaryotes