Time: Friday 22 September 15:15-16:15 Venue: Carl Kempe salen, KBC
Illuminating picornavirus replication and virus-host interactions Frank van Kuppeveld, Virology Section, Division of Infectious Diseases & Immunology, Utrecht University, The Netherlands
Although picornaviruses have been studied extensively, little is known about the processes that occur during the first several hours of infection because of a lack of sensitive assays. We developed a single-molecule imaging assay, virus infection real-time imaging (VIRIM), to study translation and replication of individual picornaviruses in live cells. VIRIM uncovered a striking heterogeneity in replication dynamics between cells and revealed extensive coordination between translation and replication of single viral RNAs.
Furthermore, we identified the replication step of the incoming viral RNA as a major bottleneck of successful infection and identify host genes that are responsible for inhibition of early virus replication. VIRIM is a powerful tool to study virus replication and virus-host interactions.