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Published: 03 Dec, 2006

New three-dimensional imaging method paves way for several research fields

NEWS A new method for 3D imaging and quantification of biological preparations ten times larger than the limit for the traditional confocal microscope is now being presented by researchers from Umeå University in Sweden in the journal Nature Methods.

Knowledge of the three-dimensional organization of tissues and organ is of key importance in most life sciences. Traditional biological imaging techniques are limited by several factors, such as the optical properties of the tissue and access to biological markers.

A major challenge in this connection has been the creation of three-dimensional images of the expressions of specific genes and proteins in large biological preparations. It has been equally complicated to try to measure the mass/volume of cells or structures that express a specific protein in a specific organ, for instance.

The study now presented, under the direction of Associate Professor Ulf Ahlgren at the Umeå Center for Molecular Medicine (UCMM, www.ucmm.umu.se), in collaboration with Dr. James Sharpe at the Centre for Genomic Regulation in Barcelona and Professor Dan Holmberg at the Department of Medical Bioscience, Umeå University, describes an elaboration of the technology for optical projection tomography (OPT) that the two former researchers helped describe in the journal Science four years ago.

The scientists have combined improvements in sample processing and tomographic data processing to develop a new method that make it possible to create 3D images of specifically dyed preparation that are one centimeter in size, of organs from adult mice and rats, for example. Moreover, the authors describe how the new method can be used to automatically measure the number and volume of specifically dyed structures in large biological preparations.

The technique requires no specially developed biological marker substances; instead, it makes use of antibodies that are in routine use in many research laboratories. The article exemplifies the potential of the technology by following the degradation of insulin-producing cells in intact pancreases from a mouse model for type-1 diabetes. In this case they demonstrate a direct connection between the volume of the remaining insulin-producing cells and the development of symptoms of diabetes.

The researchers project that it will be possible to use their method to address a great number of medical and biological issues. This may include such diverse fields as the formation of blood vessels in tumor models, the analysis of biopsies taken from patients (in cirrhosis of the liver, for instance), and autoimmune infiltration processes.

Other co-authors of the article are Tomas Alanentalo, Amir Asayesh, Christina Lorén (all UCMM) and Harris Morrison (MRC, HGU, Edinburgh).

For more information, please contact Ulf Ahlgren on ulf.ahlgren@ucmm.umu.se or phone 090-785 44 34 or 070-220 92 28.

A few examples of pictures made with the new technique:
www.umu.se/medfak/aktuellt/bilder/hjarna.jpg

Virtual clipping of OPT imaged intact mouse brain (2 days post partum) labeled for neuronal marker Isl1.
www.umu.se/medfak/aktuellt/bilder/bukspottkortlar.jpg

OPT imaged adult pancreata from a wild-type (left) and overt diabetic NOD mouse (right) labeled for insulin.
www.umu.se/medfak/aktuellt/bilder/bukspottkortel.jpg

Virtual clipping of OPT imaged intact adult mouse pancreas (wild-type) labeled for insulin. This also goes for the movie, see link below.

A portrait picture of Ulf Ahlgren is available in the Umeå University Image Database

Motion pictures made with the same tecnique is available on
ucmm.cs.it-norr.com/Research/Ulf/Bilder/adultpancreas.mov.

The film shows – for the first time – the complete distribution av the insulin-producing so called islets of Langerhans (colored red in the film) in the pancreas of an adult mouse.

Editor: Bertil Born