Our AFM can measure the processes of protein-proteins interactions such as antibody-antigen and substrate-receptor in real-time, which involves both topographic studies of the complex geometry of interacting molecules and their adhesion or recognition forces with the accuracy of nano- and pico-newton.
Location: KBC Building 5th floor Department of of Medical Biochemistry and Biophysics
The AFM system is Bruker Catalyst on inverted Ti-S Eclipse Nikon microscope (20x/0.45 and 4x/0.13 objectives, objectives can be changed upon request) and Nikon DS-L3 camera. It is equipped with broad UV fluorescence source (Lumencor) and two filters, green and orange. Microscope is also equipped with sample temperature control and perfusion pump for changing the medium/buffers.
Available cantilever stiffness range is 0.01-200 N/m, separately, the cantilevers with 130 nm tip radius (cell-stiffnes) are available for measuring the cell membrane stiffness. Experiments can be performed in both air and liquid environments. Instrument allows to measure the topography, deformation and adhesion maps and to calculate the maps of the apparent Young's modulus and dissipation. Force spectroscopy can be also performed. A range of reference materials is available allowing to use relative calibration method.