Research infrastructure DNA methylation is the process through which a methyl group is added to DNA nucleotides, most commonly at the 5-carbon position of cytosine (5mC). Methylation modifications play a crucial role in regulating gene expression by influencing the accessibility of the DNA. Changes in DNA methylation patterns can impact various cellular functions and are implicated in development, aging, and disease.
The Epigenetic Platform Umeå is located in the Translational Research Centre building in the hospital area and currently holds instrumentation for analyzing various Illumina arrays on blood, formalin-fixed paraffin-embedded (FFPE), and fresh frozen tissues. The platform at Umeå University offers operator-assisted runs and bioinformatic support for data analysis.
Instrument: Illuimna iScan
This instrument can scan a variety of arrays, including genotyping and methylation arrays. The instrument is collaboratively shared between Umeå University and Region Västerbotten.
Methylation arrays: Illumina Infinium MethylationEPIC v2.0 BeadChip.
This genome-wide methylation array targets over 935.000 CpG sites of the human methylome.
