Cryo-EM sample preparation and data collection course
The purpose of the course is to prepare and train cryoEM facility users in sample preparation methods, introduce users to the image data acquisition workflow, expand knowledge about cryo-EM methods among researchers and show that everyone can learn how to use cryo-EM for structural biology.
Image UCEM
Course description
The course is open for facility users or potential facility users, such as PhD students, postdocs, and researchers within the life sciences who are curious and will profit from cryo-EM skills. We welcome local and international applications. To attend, the course participants should be familiar with electron microscopy and structural biology.
This course is supported by SciLifeLab, CryoNET and Umeå University and organized by the Umeå Centre for Electron Microscopy (UCEM).
Course content
This course will cover practical aspects of cryo-EM sample preparation and data acquisition.
The following topics will be covered:
Overview of cryo-EM methods
Microscope design and electron optics
Sample preparation
Data collection for single particle analysis (SPA)
Introduction to SPA image processing
The course consists of lectures, cryo lab work, demonstrations and practical exercises for all participants, hands-on experiences of microscopy operation and discussions. A detailed schedule will be provided to accepted participants.
Course aim and literature
The aim of the course is for the participants to increase their knowledge about cryogenic electron microscopy methods, data collection and analysis, and acquire practical skills in sample preparation for cryo-EM.
Course literature includes handouts and online resources.
Entry requirements
Priority will be given to researchers involved in a project where the use of cryo-EM is needed, which are to be briefly described upon application. The course is suitable for researchers in the life sciences, with a masters or PhD degree, including graduate students, postdocs and staff scientists. A maximum of 18 participants will be admitted. Please provide a clear description of your research project and motivation for attending the course.
Registration
Application should be submitted before November 1st, 2026.
Registration form
Location
Glasburen, KBC - Link to MAZEMAP UCEM and Chemical Biological Centre (KBC) Building Umeå University Linnaeus väg 6
Teachers and course instructors
Linda Sandblad
Ieva Ragaisyte
Lorene Gonnin
Tanvir Shaikh
Erin Schexnaydre
Additional teachers t.b.a
Examination
Participation in all parts of the course, lectures, lab, microscopy demonstrations and evaluation are mandatory. Upon completion, SciLifeLab and Umeå University will provide a course certificate and a recommendation for 1 ECTS.