Research infrastructure Our fluorescence microscopy is a top-end inverted microscope that can be used to look at a sample through a transparent substrate
Our fluorescence microscopy is a top-end inverted microscope that can be used to look at a sample through a transparent substrate. Operation in “standard” epifluorescence mode with up to 6 different colors, allows the light to penetrate the sample and provides excellent colocalization of different fluorescent signals within a sample. Additionally, this microscope is equipped to operate in a Total Internal-Reflection Fluorescence (TIRF) mode (available in 488 & 561 nm) which makes it possible to image fluorescently labeled objects within ~200nm of the coverslip, thereby providing discrimination against signals in the bulk of the sample. TIRF is ideal for observing fluorescently labeled species adsorbing to a functuonalized glass surface or for observing the adherent membrane of cells and some intercellular structures. The microscope is also equipped with a fluorescence recovery after photobleaching setup, which makes it possible to etermine the diffusion of fluorescence molecules in the sample (e.g. through cells or on the glass surface). This microscope part of the BICU infrastructure. More information can be found on https://www.umu.se/en/research/infrastructure/biochemical-imaging-centre-umea-bicu/light-microscopy/ (scroll down to Nikon widefield/TIRF)
