Research infrastructure A facility for performing experiments under hypoxic conditions has been created at the Molecular Biology Department at Umeå University.
Chemostate BioFlo 120 (hypoxic/anoxic) is a 2 L bench-scale bioreactor/fermentor system perfectly suited for all levels of research and development. The system was designed to be flexible to meet the wide-ranging needs of scientists today. Digital Mettler Toledo® ISM sensor technology is fully integrated allowing the user to choose between pH, redox, and dissolved oxygen (polarographic and/or optical), measurements without the need for additional equipment.
Image Eppendorf AG
The H135 HEPA is a tall, wide, deep hypoxic chamber. This Hypoxystation has a capacity of 900 litres and can accommodate a variety of items of equipment such as live cell imaging devices, microscopes, plate readers, etc.
Image Don Whitley Scientific Limited, BioTek Instruments, Inc.
The H35 Hypoxystations creates hypoxic and anoxic conditions within a controlled and sustained workstation environment. It is ideal for cell and tissue culture researchers wanting to accurately control oxygen, carbon dioxide, temperature and humidity. Applications for this instrument include cancer research, neurology and cardiovascular research, as well as a many other types of cell culture work.
Image Don Whitley Scientific Limited
The Whitley DG250 Anaerobic Workstation is used when there is a need to easily process, culture and examine anaerobic samples without exposure to atmospheric oxygen. This workstation has a small footprint yet can accommodate 270 x 90mm Petri dishes whilst retaining a generous working area.
Image Don Whitley Scientific Limited
Anoxomat is a system that easily creates repeatable environments with hypoxic and anoxic conditions for your growth plates.
Image I&L Biosystems
The S3e cell sorter is equipped with two lasers and four fluorescent detectors, plus both forward and side scatters. Samples are analyzed using the traditional jet-in-air technology, and events can be sorted at high speeds while maintaining sensitivity and high-purity. The S3e cell sorter is largely efficient and user-friendly by automating the complex presort setup with a unique technology (ProDrop technology).
Image Bio-Rad Laboratories, Inc.
Excella e25 incubator
Image Eppendorf AG
Eppendorf refrigerated centrifuges
Image Eppendorf AG
The A135 HEPA is a tall, wide, deep anoxic chamber where we have accommodated an upright Zeiss fluorescence microscope. In this chamber we can monitor organisms' growth or protein's location in real-time under controlled oxygen concentrations.
Image Emilio Bueno
The Whitley M85 Workstation is an extremely flexible system as it allows users to programme precise gas concentrations and use the workstation with anoxic/anaerobic, microaerobic or hypoxic atmospheres without the need to change incoming gases.
Image Don Whitley Scientific Limited
Chemostate BioFlo 120 (hypoxic/anoxic) is a 2 L bench-scale bioreactor/fermentor system perfectly suited for all levels of research and development. The system was designed to be flexible to meet the wide-ranging needs of scientists today. Digital Mettler Toledo® ISM sensor technology is fully integrated allowing the user to choose between pH, redox, and dissolved oxygen (polarographic and/or optical), measurements without the need for additional equipment.
The H135 HEPA is a tall, wide, deep hypoxic chamber. This Hypoxystation has a capacity of 900 litres and can accommodate a variety of items of equipment such as live cell imaging devices, microscopes, plate readers, etc.
The H35 Hypoxystations creates hypoxic and anoxic conditions within a controlled and sustained workstation environment. It is ideal for cell and tissue culture researchers wanting to accurately control oxygen, carbon dioxide, temperature and humidity. Applications for this instrument include cancer research, neurology and cardiovascular research, as well as a many other types of cell culture work.
The Whitley DG250 Anaerobic Workstation is used when there is a need to easily process, culture and examine anaerobic samples without exposure to atmospheric oxygen. This workstation has a small footprint yet can accommodate 270 x 90mm Petri dishes whilst retaining a generous working area.
Anoxomat is a system that easily creates repeatable environments with hypoxic and anoxic conditions for your growth plates.
The S3e cell sorter is equipped with two lasers and four fluorescent detectors, plus both forward and side scatters. Samples are analyzed using the traditional jet-in-air technology, and events can be sorted at high speeds while maintaining sensitivity and high-purity. The S3e cell sorter is largely efficient and user-friendly by automating the complex presort setup with a unique technology (ProDrop technology).
Excella e25 incubator
Eppendorf refrigerated centrifuges
The A135 HEPA is a tall, wide, deep anoxic chamber where we have accommodated an upright Zeiss fluorescence microscope. In this chamber we can monitor organisms' growth or protein's location in real-time under controlled oxygen concentrations.
The Whitley M85 Workstation is an extremely flexible system as it allows users to programme precise gas concentrations and use the workstation with anoxic/anaerobic, microaerobic or hypoxic atmospheres without the need to change incoming gases.
Felipe Cava and Emilio Bueno at Umeå Hypoxic Research Facility
ImageDavid ArranzUHRF is a facility for working under controlled oxygen concentrations with eukaryotic and prokaryotic organisms. It consists of 5 hypoxic/anoxic workstations (Don Whitley Scientific): 2x H35, 1x H135 (with HEPA filter and an Epoch2 plate reader), 1x DG250 workstation, and an Anoxomat (Advanced Instruments). In addition, we have a chemostate (Bioflo 120, Eppendorf) for microbial growth under highly controlled conditions, a cell sorter (Biorad S3), and several incubators, shakers and refrigerated centrifuges. A yearly survey will collect user’s feedback to guide the incorporation of future equipment.
Please note that policies and rules may be updated. To ensure you have the most recent policy, contact Emilio Bueno or Felipe Cava.
Last update 2021-01-28:
The User Club membership provides access to all the equipment present in the facility with a yearly fee of 10,000 SEK. In addition, there will be a fee for each user per year depending on the estimated number of times that the facility will be used (see Services fees below).
For use of any equipment at UHRF it is mandatory to take the corresponding training introduction. If users are found to lack appropriate training introduction for the equipment, access to the facility will be terminated for the user and the corresponding group and PI.
If the instrument is damaged due to its incorrect use, the Principal Investigator will be charged accordingly.
The Principal Investigator must provide a list of people from the lab that will have access to the instrument in order to get access to the room.
Services from the UHRF includes training courses, scientific support/recommendation, cleaning of the equipment, continuous gases supply and weekly calibration of the instruments.
The agreement for use of UHRF must be renewed every year.
Please note that fees may be updated. To ensure you have the most recent fees, contact Emilio Bueno or Felipe Cava.
User club fee (per lab/year):
All instruments: 10,000 SEK
Training and License (one-time fee per user):
All instruments: 300 SEK/person
Booking fees:
Manipulation time:
H135 (hypoxic): 20 SEK/h
H35 (hypoxic): 20 SEK/h
DG250 (anoxic): 20 SEK/h
Chemostate: 200 SEK/day
Cell sorter: 350 SEK/h
Long-term incubations:
H135 (hypoxic): 14 SEK/h
H35anox and A35 (Anoxic): 14 SEK/h
DG250 (anoxic): 14 SEK/h
Anoxomat: 1 SEK/h
UHRF is located in room 6L-004 on floor -1 in the Department of Molecular Biology.
If you need to visit Emilio Bueno's office, it is located in room 6K-10 on floor 0. His working hours are from 9.30 to 13.30.
Find seminars, courses, workshops, and symposiums related to Umeå Hypoxia Research Facility listed here.
Emilio Bueno and Felipe Cava using two H35 Hypoxystations.
ImageDavid ArranzTo book some of the instruments available in the UHRF, visit the link below and log in with your own lab user/password and book your dates.*
Booking of instruments in the UHRF
Please note that booking fees may be updated. To ensure you have the most recent booking fees, contact Emilio Bueno or Felipe Cava.
Daily use:
H135 (hypoxic): 35 SEK/h
H35 (hypoxic): 20 SEK/h
DG250 (anoxic): 15 SEK/h
Chemostate: 200 SEK/day
Cell sorter: 35 SEK/h
Long-term incubations:
H135 (hypoxic): 1 SEK/h
H35 (hypoxic): 1 SEK/h
DG250 (anoxic): 1 SEK/h
Anoxomat: 1 SEK/h
*Cancellation policy: The entire fee will be charged if the booking is not cancelled 24h ahead of use.
