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Staff photo Andrei Chabes

Andrei Chabes

dNTPs and maintenance of genome stability

Contact

Works at

Affiliation
Professor at Department of Medical Biochemistry and Biophysics Role: Directory Co-ordinator, Head of department
Location
KBC building, H6, Campus, Umeå, A6-42-21 Umeå universitet, 901 87 Umeå
Affiliation
Affiliated as professor at Molecular Infection Medicine Sweden (MIMS)
Location
Medicinsk kemi och biofysik, 90187 Umeå

dNTPs and maintenance of genome stability

The four dNTPs (dATP, dTTP, dCTP, and dGTP) are the building blocks of DNA. A balanced supply and a correct overall concentration of dNTPs are key prerequisites for faithful genome replication. These requirements, along with the fact that the concentrations of dNTPs fluctuate during the cell cycle, mean that the production of dNTPs must be tightly regulated through multiple mechanisms.

We are investigating (i) how the genome integrity checkpoint regulates the concentration of dNTPs and how dNTPs regulate the activation of the genome integrity checkpoint, and (ii) how imbalanced dNTP pools affect the fidelity of replication and genome stability and how different replication errors are recognized and repaired. We hope to understand how metabolic changes in the dNTP pool balance affect aging, the development of cancer, and other genetic disorders.

Our latest publications:

Wanrooij PH, Tran P, Thompson LJ, Carvalho G, Sharma S, Kreisel K, Navarrete C, Feldberg AL, Watt DL, Nilsson AK, Engqvist MKM, Clausen AR and Chabes A (2020) Elimination of rNMPs from mitochondrial DNA has no effect on its stability. PNAS 117:14306-14313  

Sushma S, Koolmeister C, Tran P, Nilsson AK, Larsson NG and Chabes A (2020) Proofreading deficiency in mitochondrial DNA polymerase does not affect total dNTP pools in mouse embryos. Nature Metabolism  doi.org/10.1038/s42255-020-0264-z

All publications in PubMed (by date) and in Google Scholar (by citations).

Nucleic Acids Research, Oxford Academic 2020, Vol. 48, (8) : 4274-4297
Cerritelli, Susana M; Iranzo, Jaime; Sharma, Sushma; et al.
Comptes rendus. Biologies, Académie des Sciences 2020, Vol. 343, (1) : 9-21
Coquel, F.; Silva, M. J.; Techer, H.; et al.
Cell reports, Elsevier 2020, Vol. 31, (6)
Davenne, Tamara; Klintman, Jenny; Sharma, Sushma; et al.
Molecular Cell, Elsevier 2020, Vol. 78, (3) : 496-410.e4
Forey, Romain; Poveda, Ana; Sharma, Sushma; et al.
Nucleic Acids Research, Oxford University Press 2020, Vol. 48, (1) : 264-277
Schmidt, Tobias T; Sharma, Sushma; Reyes, Gloria X; et al.
Nature Metabolism, Nature Publishing Group 2020, Vol. 2, (8) : 673-675
Sharma, Sushma; Koolmeister, Camilla; Tran, Phong; et al.
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences 2020, Vol. 117, (25) : 14306-14313
Wanrooij, Paulina H.; Tran, Phong; Thompson, Liam J.; et al.
FEBS Open Bio, WILEY 2019, Vol. 9 : 21-21
Chabes, Andrei
Nucleic Acids Research, Oxford University Press 2019, Vol. 47, (8) : 3986-3995
Garbacz, Marta A.; Cox, Phillip B.; Sharma, Sushma; et al.
Molecular Cell, Elsevier 2019, Vol. 76, (5) : 784-+
Nicholls, Thomas J.; Spåhr, Henrik; Jiang, Shan; et al.
PLoS ONE, Public Library of Science 2019, Vol. 14, (3)
Rentoft, Matilda; Svensson, Daniel; Sjödin, Andreas; et al.
Nucleic Acids Research, Oxford University Press 2019, Vol. 47, (1) : 237-252
Schmidt, Tobias T.; Sharma, Sushma; Reyes, Gloria X.; et al.
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology 2019, Vol. 394, (44) : 15889-15897
Tran, Phong; Wanrooij, Paulina H.; Lorenzon, Paolo; et al.
FEBS Letters, John Wiley & Sons 2019, Vol. 593, (13) : 1554-1565
Wanrooij, Paulina H.; Chabes, Andrei
Nature Communications, Nature Publishing Group 2019, Vol. 10
Xing, Xuanxuan; Kane, Daniel P.; Bulock, Chelsea R.; et al.
EMBO Journal, Wiley-VCH Verlagsgesellschaft 2018, Vol. 37, (21)
Bacal, Julien; Moriel-Carretero, María; Pardo, Benjamin; et al.
Nature, Vol. 557, (7703) : 57-61
Coquel, Flavie; Silva, Maria-Joao; Técher, Hervé; et al.
Nucleic Acids Research, Oxford University Press 2018, Vol. 46, (11)
Kong, Ziqing; Jia, Shaodong; Chabes, Anna Lena; et al.
Genes & Development, Vol. 32, (11-12) : 822-835
Lanz, Michael Charles; Oberly, Susannah; Sanford, Ethan James; et al.
EMBO Journal, Wiley-VCH Verlagsgesellschaft 2018, Vol. 37, (17)
Li, Shuqi; Xu, Zhiyun; Xu, Jiawei; et al.
PLoS Genetics, PUBLIC LIBRARY SCIENCE 2018, Vol. 14, (1)
Peng, Xiao P.; Lim, Shelly; Li, Shibai; et al.
G3: Genes, Genomes, Genetics, Vol. 8, (8) : 2551-2558
van Mourik, Paula M; de Jong, Jannie; Sharma, Sushma; et al.
Science, American Association for the Advancement of Science 2018, Vol. 361, (6409) : 1386-+-
Yu, Chuanhe; Gan, Haiyun; Serra-Cardona, Albert; et al.
Molecular Cell, Elsevier 2017, Vol. 68, (2) : 446-455
Gan, Haiyun; Yu, Chuanhe; Devbhandari, Sujan; et al.
Oncogene, Macmillan Publishers Ltd. 2017, Vol. 36, (42) : 5808-5818
Garzón, J.; Rodríguez, R.; Kong, Ziqing; et al.
Nucleic Acids Research, Vol. 45, (3) : 1200-1218
Kochenova, Olga V; Bezalel-Buch, Rachel; Tran, Phong; et al.
PLoS Genetics, Vol. 13, (10)
Maicher, André; Gazy, Inbal; Sharma, Sushma; et al.
Journal of Hepatology, ELSEVIER SCIENCE BV 2017, Vol. 66, (1) : S321-S321
Nystrom, K.; Pettersson, G.; Wanrooij, Paulina; et al.
Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, (22) : E4442-E4451
Schmidt, Tobias T; Reyes, Gloria; Gries, Kerstin; et al.
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences 2017, Vol. 114, (47) : 12466-12471
Wanrooij, Paulina H.; Engqvist, Martin K. M.; Forslund, Josefin M. E.; et al.
Nucleic Acids Research, Vol. 44, (9) : 4222-4232
Kenigsberg, Ephraim; Yehuda, Yishai; Marjavaara, Lisette; et al.
Cell reports, Vol. 17, (6) : 1657-1670
Liew, Li Phing; Lim, Zun Yi; Cohen, Matan; et al.
Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, (17) : 4723-4728
Rentoft, Matilda; Lindell, Kristoffer; Tran, Phong; et al.
Nucleic Acids Research, Vol. 44, (4) : 1669-1680
Watt, Danielle L.; Buckland, Robert J.; Lujan, Scott A.; et al.
OncoTarget, Vol. 6, (14) : 12587-12602
Barfeld, Stefan J; Fazli, Ladan; Persson, Margareta; et al.
Yeast, Wiley-Blackwell 2015, Vol. 32, (Suppl. 1) : S56-S56
Earp, Caroline; Rowbotham, Samuel; Marjavaara, Lisette; et al.
Biology open, Vol. 4, (12) : 1739-1743
Earp, Caroline; Rowbotham, Samuel; Merényi, Gábor; et al.
DNA Replikation: Methods and Protocols, New York: Springer-Verlag New York 2015 : 113-121
Jia, Shaodong; Marjavaara, Lisette; Buckland, Robert; et al.
Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, (19) : E2467-E2476
Mertz, Tony M; Sharma, Sushma; Chabes, Andrei; et al.
Nature Structural & Molecular Biology, Vol. 22, (4) : 291-U35
Williams, Jessica S; Clausen, Anders R; Lujan, Scott A; et al.
Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, (19) : E2457-E2466
Williams, Lindsey N; Marjavaara, Lisette; Knowels, Gary M; et al.
PLOS Genetics, Vol. 10, (12)
Buckland, Robert J; Watt, Danielle L; Chittoor, Balasubramanyam; et al.
Scientific Reports, Macmillan Publishers Ltd. 2014, Vol. 4 : 5539-
Domkin, Vladimir; Chabes, Andrei
PLOS Genetics, Public library science 2014, Vol. 10, (10) : e1004667-
Lin, Chia-Yeh; Wu, Meng-Ying; Gay, Sophie; et al.
Nucleic Acids Research, Oxford University Press 2014, Vol. 42, (10) : 6314-6325
Rubinstein, Linda; Ungar, Lior; Harari, Yaniv; et al.
Molecular Cell, Vol. 54, (4) : 691-697
Yoshida, Kazumasa; Bacal, Julien; Desmarais, Damien; et al.
Molecular Cell, Vol. 56, (4) : 551-563
Yu, Chuanhe; Gan, Haiyun; Han, Junhong; et al.
Genetics, The Genetics Society 2013, Vol. 193, (4) : 1095-1105
Gupta, Amitabha; Sharma, Sushma; Reichenbach, Patrick; et al.
Molecular and Cellular Biology, Vol. 33, (16) : 3202-3213
Hoch, Nicolas C; Chen, Eric S-W; Buckland, Robert; et al.
Nucleic Acids Research, Oxford University Press 2013, Vol. 41, (22) : 10371-10378
Tsaponina, Olga; Chabes, Andrei

Research groups

Head of research
Andrei Chabes