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Biochemistry: Protein Separation Methods and Analytical Techniques, 15 Credits

Swedish name: Proteinkemisk separations- och analysteknik

This syllabus is valid: 2018-01-22 and until further notice

Course code: 5KE171

Credit points: 15

Education level: Second cycle

Main Field of Study and progress level: Chemistry: Second cycle, in-depth level of the course cannot be classified

Grading scale: Three-grade scale

Responsible department: Department of Chemistry

Established by: Faculty Board of Science and Technology, 2017-06-15

Revised by: Faculty Board of Science and Technology, 2018-01-16


Purification and analysis of proteins is an area that grows both in academic and industry research. The area requires good knowledge not only in protein chemistry but also in nearby areas such as genetic engineering, chromatography, immunology and spectroscopic methods. The course covers the theoretical basics for using and interpreting results from modern protein separation methods. It also aims to provide such in-depth knowledge in experimental protein chemistry that the student can analyze protein properties. The laboratory part constitutes the major part of the course and provides training in skills, training in planning and carrying out the laboratory work independently, and evaluating and presenting their results both orally and in written form.

Expected learning outcomes

After completing the course, the student should be able to:

  • plan and carry out the separation of proteins independently
  • analyze critically and evaluate the results, which are obtained from experimental protein separation
  • use scientific articles, encyclopedias and databases to retrieve information and critically review, summarize and account for its content
  • know how to choose and use and different methods of protein analysis
  • optimize purification strategies and purification hygiene in the choice of protein purification strategy
  • perform laboratory analyzes according to Good Laboratory Practice (GLP)
  • demonstrate an understanding of the various purity requirements and analyzes that exist in different parts of the protein chemical industry
  • compile and process experimental data, and present them orally and in written form
  • participate actively in discussions at seminars and at group exercises

Required Knowledge

90 ECTS credits including the course Biochemistry 15 ECTS credits (5KE020/075) or Biochemistry,  Protein Chemistry and Enzyme Kinetics 15 ECTS credits (5KE076) or equivalent and English B/6.

Form of instruction

The teaching is conducted in the form of lectures, lessons, group exercises, demonstrations and laboratory exercises. The laboratory assignments and associated group exercises are mandatory.

Examination modes

The examination takes place partly by a written and an oral exam on the theoretical part, and partly by an oral and a written presentation of the laboratory work. For the performance in a written and oral exam, and in the laboratory work, one of the following grades is given: Fail (U), Pass (G), and Excellent (VG). On the whole course one of the following grades is given: Fail (U), Pass (G), and Excellent (VG). To pass the entire course, all examinations and compulsory tasks must be fulfilled. The assessment is a combined assessment of the results of the various parts of the examination. For the grade VG, VG is required for all examinations. Students who do not pass the regular exam date are entitled to a new test. Students who have failed more than twice in a test are entitled to request that another examiner is appointed by the head of the Department of Chemistry.
Examination on this course plan is guaranteed two years after the student's first-time registration on the course.
Allocations (of credits) are always assessed individually (see the university's rules)


The literature list is not available through the web. Please contact the faculty.