Cryo-focused ion beam milling (cryo-FIB-SEM): Taking you where you need to go
Tue
6
May
Tuesday 6 May, 2025at 12:00 - 13:00
Glasburen, KBC, and Zoom
In the frame of the “SciLifeLab Site Umeå / KBC Infrastructure Seminar Series”, the seminars will be organised on Tuesdays during spring 2025. The seminars will be held in a hybrid format - IRL in Glasburen, KBC, Umeå, and online via Zoom. Learn more about the seminar series and Schedule for Spring 2025here.
On 6 May, at 12:00, SciLifeLab Site Umeå / KBC Infrastructure Seminar Series presents:
"Cryo-focused ion beam milling (cryo-FIB-SEM): Taking you where you need to go"
Speaker: Erin Schexnaydre, Staff scientist, Umeå Centre for Electron Microscopy (UCEM), Umeå University
Short description: Cryo-focused ion beam milling (cryo-FIB) is a powerful technique. Its power comes from its versatility to study biological systems in vitro or in situ, i.e. within cells or tissue. It has the ability to reach uncharted regions in biological systems. Cryo-FIB milling is used to expose 100–250 nm thin sections (called lamella) in intact frozen cells and tissue. The process allows access into the cells for high-resolution cryo-electron tomography (cryo-ET) and structure determination of biomolecules in their native environment.