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The cryo-electron tomography workflow featuring scanning electron images of a cellular lamella and one slice from a transmission electron tomogram from a lamella

Cryo-focused ion beam milling (cryo-FIB-SEM): Taking you where you need to go

Tue
6
May
Time Tuesday 6 May, 2025 at 12:00 - 13:00
Place Glasburen, KBC, and Zoom

In the frame of the “SciLifeLab Site Umeå / KBC Infrastructure Seminar Series”, the seminars will be organised on Tuesdays during spring 2025. The seminars will be held in a hybrid format - IRL in Glasburen, KBC, Umeå, and online via Zoom. Learn more about the seminar series and Schedule for Spring 2025 here.

On 6 May, at 12:00, SciLifeLab Site Umeå / KBC Infrastructure Seminar Series presents:

"Cryo-focused ion beam milling (cryo-FIB-SEM): Taking you where you need to go"

 

Speaker: Erin Schexnaydre, Staff scientist, Umeå Centre for Electron Microscopy (UCEM), Umeå University

Short description: Cryo-focused ion beam milling (cryo-FIB) is a powerful technique. Its power comes from its versatility to study biological systems in vitro or in situ, i.e. within cells or tissue. It has the ability to reach uncharted regions in biological systems. Cryo-FIB milling is used to expose 100–250 nm thin sections (called lamella) in intact frozen cells and tissue. The process allows access into the cells for high-resolution cryo-electron tomography (cryo-ET) and structure determination of biomolecules in their native environment.

Webpage of Umeå Centre for Electron Microscopy (UCEM)

Registration: Sign up for the IRL seminar and reserve your free lunch (salad, drink, and a little sweet snack) by Friday, 2 May, 13:30:

https://www.umu.se/en/research/infrastructure/scilifelab/scilifelab-site-umea--kbc-infrastructure-seminar-series/

or follow online (registration is not required):

Meeting URL: https://umu.zoom.us/j/69390567429

Organisers: SciLifeLab Site Umeå and Chemical Biological Centre (KBC), Umeå

 

Organiser: KBC
Event type: Seminar
Staff photo Erin Schexnaydre
Speaker
Erin Schexnaydre
Staff scientist
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Anna Shevtsova
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Rebecca Forsberg
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