Currently there are two courses given at CBCS. One is a web-based theoretical course, giving a basic introduction to high throughput screening. The other is a one week practical course given in collaboration with LCBKI. The theoretical course is a prerequisite for taking the practical course.
The course gives a broad introduction to high throughput screening (HTS) that is technique used to identify biologically active small organic molecules through screening of compound libraries with the help of robust test systems and advanced instrumentation.
Participants can start at any time and study at their own pace since the course is entirely web-based. The course material is in English consisting of text and explanatory figures, video lectures, review articles, and movies.
Responsible department: Department of Chemistry Level: Advanced
Content: The course includes a description of small organic molecules as research tools and starting points for drug development. In addition different parameters to consider when designing a compound collection for HTS will be reviewed. Biological activity is most cases that the organic molecule affect a protein or other biomolecule. The course describes development of robust and statistically validated test systems that allow biological activity to be measured by e.g. luminescence, absorbance, or fluorescence. The most common instruments for liquid handling and reading of the test system signal will be reviewed. The course also covers how HTS is performed and how molecules that are identified as potentially actives are followed up by analysis of analogs and additional test systems. HTS generates large amounts of data and the course briefly reviews the important role that an informatics system has. The course also covers a number of special topics and case studies to illustrate the application of HTS.
Prerequisites: Bachelor degree (120 ECTS) in chemistry, molecular biology, pharmacology or equivalent. English knowledge corresponding to the Swedish upper secondary education English B.
Registration Please send an E-mail to Stina Berglund Fick if you want to register for the course.
Introduction to HTS course details (pdf)
The course gives a practical basis for assay development in high throughput screening. The course is given as complement to the theoretical on-line course "Introduction to high throughput screening" (see above). Passed examination of the theoretical course is a prerequisite for taking the practical.
Venue: Laboratories for Chemical Biology Umeå (LCBU), Umeå University, Umeå
Responsible department: Department of Chemistry, Umeå University Level: Advanced.
Content: The course gives a practical basis for assay development in high throughput screening and is given as a practical complement to the course "Introduction to high throughput screening". High throughput screening (HTS) is a technique used to identify biologically active small organic molecules through screening of compound libraries with the help of robust test systems and advanced instrumentation. The course includes several aspects of assay development applicable to both target-based and cell-based assays, however, the focus will be on cell-based assays. The assay carried out in the course includes cell culturing and bacterial infection, thus experience in cell culturing and good microbial lab practice is required.
Parameters of importance for optimization of a cell based assay will be tested empirically in the laboratory and their significance calculated using Z'. During the course a small-scale screen will be executed. The students will thereby be given knowledge about the importance of good screening logistics and gain insight into the amount of data generated and handling thereof. During the course the students will be introduced to instrumentation commonly used in high throughput screening such as liquid handling instrumentation, plate readers and high content screening microscope.
Prerequisites: Bachelor degree (120 ECTS) in chemistry, molecular biology, pharmacology or equivalent. English knowledge corresponding to the Swedish upper secondary education English B. Practical experience in cell culturing and good microbial practice. The theoretical course: Introduction to high throughput screening (2 ECTS)
Register: by sending an e-mail Stina Berglund Fick. Include a letter with motivation to take the course. The number of participants is limited.