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CLEM instruments

FEI Titan Krios TEM

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  • Location:
    UCEM facility in KBC building - Map to UCEM can be found here

  • Technical Specification:

    • Accelerating voltage: Operating at 300 kV

    • Electron source: X-FEG

    • Detector: Ceta 4k x 4k CMOS, Falcon3 4k x 4k direct electron detector, Gatan K2 BioQuantum (energy filter) 4k x 4k direct electron detector

    • Configurations: Autoloder for 12 grids, Volta phase plate, data collection at cryo conditions

    • Data acquisition software: TIA, EPU, Tomography, Gatan Digital Micrograph, SerialEM

Scios DualBeam (FIB-SEM)

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  • Location:
    UCEM facility in KBC building - Map to UCEM can be found here

  • Technical Specification:

    • Accelerating voltage: 20 V - 30 kV (EB); 500 V - 30 kV (IB)

    • Electron emitter: Thermal field emission

    • Ion emitter: Ga LMIS

    • Resolution (optimal WD): 1.6 nm @ 1 kV

    • Detector: Trinity T1/T2, ET-SE, ICE, DBS, CCD, Nav-Cam

    • Stage configuration: 5-axes eucentric motorized

    • System control: xT GUI with additional options (GIS, Micromanipulator, Plasma coater, ASV, AutoFIB, AutoTEM)

    • System mode: High-vacuum @ RT or cryogenic temperature

 

FEI Talos L120C

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  • Location:
    UCEM facility in KBC building - Map to UCEM can be found here

  • Technical Specification:

    • Accelerating voltage: Operating at 120 kV

    • Electron source: LaB6 filament

    • Detector: Ceta 4k x 4k CMOS

    • Data acquisition software: Velox, Tomography, Maps and SerialEM

    • Tomography high tilt holder -70 to 70 °

    • Gatan 626 cryo holder

Leica Cryo Thunder

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Wide-field light microscope for imaging and analysis of Cryo-samples which can be further processed for Electron microscopy analysis.

  • Location:
    UCEM facility in KBC building - Map to UCEM can be found here
  • Technical Specification:
    • Leica DM6 FS
    • Cryo objective (0.28 mm): 50x/0.9
    • 4.2 MP sCMOS camera
    • Motorised Cryo-stage
    • Cryo transfer shuttle
    • Filter sets:
        DAPI - Ex 350/50, D 400(LP), Em 460/50
        GFP -  Ex 470/40, D 495, Em 525/50
        TXR -  Ex 560/40, D 585 (LP), Em 630/75

Carl Zeiss Merlin FESEM

Field Emission Scanning Electron Microscope (FE-SEM) from Carl Zeiss

More Details

  • Location:
    Level 5 KBC - C Building - Map to KBC can be found here

  • Technical Specification:
    • Accelerating voltage: 20 V - 30 kV
    • Electron emitter: Thermal field emission
    • Resolution (optimal WD): 1.4 nm @ 1 kV; 0.6 nm @ 30 kV (STEM mode)
    • Detector: In-lens SE, ET-SE, BSD, EsB, STEM, CCD, EDS
    • Stage configuration: 5-axes eucentric motorized
    • System control: SmartSEM GUISystem mode: High-vacuum @ RT or cryogenic temperature

Leica Widefield Thunder

More Details

  • Location:
    KBC Building 6th floor Department of Medical Biochemistry and Biophysics.
  • Room:
    A6 44 15
  • Price:
    150kr/hr - Driver's License 2000kr
  • Objectives:
    • N PLAN 5x/0.12 PH0
    • HC PL FLUOT AR 10x/0.32 PH1
    • HC PL FL L 20x/0.40 CORR PH1
    • HC PL APO 20x/0.80
    • HC PL FLUOTAR L 40x/0.60 CORR
    • HC PL APO 40x/0.95 CORR
    • HC PL APO 63x/1.40-0.60 OIL
    • HC PL FLUOTAR 100x/1.32 OIL PH3
  • Techniques:
    • Widefield
    • Live cell imaging
    • Computational clearance: Thunder
Latest update: 2022-08-19