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Cryo-EM

FEI Titan Krios TEM

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  • Location:

UCEM facility in KBC building - Map to UCEM can be found here

  • Technical Specification:

- Accelerating voltage: Operating at 300 kV
- Electron source: X-FEG
- Detector: Ceta 4k x 4k CMOS, Falcon3 4k x 4k direct electron detector, Gatan K2 BioQuantum (energy filter) 4k x 4k direct electron detector
- Configurations: Autoloder for 12 grids, Volta phase plate, data collection at cryo conditions
- Data acquisition software: TIA, EPU, Tomography, Gatan Digital Micrograph, SerialEM

FEI Glacios

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Location:
UCEM facility in KBC building - Map to UCEM can be found here

Technical Specification:
- Accelerating voltage: 80–200 kV
- Electron emitter: High-brightness field emission gun (X-FEG)
- Resolution: Symmetric Constant-PowerTM C-TWIN objective lens, combining excellent resolution with high tilt and cryo capabilities (focal length = 3.5 mm; Cs = 2.7 mm, Cc = 2.7 mm)
- Detector: Falcon 4i Direct Electron Detector - HAADF STEM detector - On-axis BF/DF detectors
- Stage configuration: Computerized 4-axes specimen stage with ±70° alpha tilt - Cryo-stage with single axis holder

Scios DualBeam (FIB-SEM)

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  • Location:

UCEM facility in KBC building - Map to UCEM can be found here

  • Technical Specification:

- Accelerating voltage: 20 V - 30 kV (EB); 500 V - 30 kV (IB)
- Electron emitter: Thermal field emission
- Ion emitter: Ga LMIS
- Resolution (optimal WD): 1.6 nm @ 1 kV
- Detector: Trinity T1/T2, ET-SE, ICE, DBS, CCD, Nav-Cam
- Stage configuration: 5-axes eucentric motorized
- System control: xT GUI with additional options (GIS, Micromanipulator, Plasma coater, ASV, AutoFIB, AutoTEM)
- System mode: High-vacuum @ RT or cryogenic temperature

 

FEI Talos L120C

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  • Location:

UCEM facility in KBC building - Map to UCEM can be found here

  • Technical Specification:

- Accelerating voltage: Operating at 120 kV
- Electron source: LaB6 filament
- Detector: Ceta 4k x 4k CMOS
- Data acquisition software: Velox, Tomography, Maps and SerialEM
- Tomography high tilt holder -70 to 70 °
- Gatan 626 cryo holder

Leica Cryo Thunder

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Wide-field light microscope for imaging and analysis of Cryo-samples which can be further processed for Electron microscopy analysis.

  • Location:
    UCEM facility in KBC building - Map to UCEM can be found here
  • Technical Specification:
    - Leica DM6 FS
    - Cryo objective (0.28 mm): 50x/0.9
    - 4.2 MP sCMOS camera
    - Motorised Cryo-stage
    - Cryo transfer shuttle
    - Filter sets:
      DAPI - Ex 350/50, D 400(LP), Em 460/50
      GFP -  Ex 470/40, D 495, Em 525/50
      TXR -  Ex 560/40, D 585 (LP), Em 630/75

Cryo-EM Specimen Preparation

Vitrobot

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  • Technical Specification:

Vitrobot completely automates the vitrification process to provide fast, easy, reproducible sample preparation - the first step in obtaining high quality images and repeatable experimental results.
- User-defined settings ensure maximum flexibility
- Fully automated and reproducible vitrification of suspensions
- High vitrification quality through controlled environment
- High sample throughput
- An effective user interface assures easy and flexible instrument control

Glow discharge PELCO easiGlow

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  • Technical Specification:

For one of the most common glow discharge applications, making TEM support films or grids hydrophilic using air, the PELCO easiGlow™ uses an automated and quick cycle with fully selectable parameters. The microprocessor controlled system also offers full manual control for all parameters and an advanced protocol programming feature for custom designed glow discharge applications. Parameters and protocols can be stored to facilitate consistent glow discharge results. Open the shipping box, plug it in, and you can start.

- Optimized for cleaning TEM grids
- Precise and easy vacuum setting
- Short cycle times
- Consistent results
- Auto, programmed and manual mode
- Intuitive touch screen for display and operation
- Supports hydrophilic/hydrophobic and negative/positive modes
- Affordable, compact, standalone system