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Sample preparation equipment

SEM Preparation Equipment:

Gatan PECS II Broad Ion Beam Miller

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  • Technical Specification:

Fully automated argon ion polishing system suitable for preparation of SEM samples to prepare damage free surfaces, cross sections and deposit coatings to protect or eliminate charging.

- Polish, etch or coat samples with a single pump down.
- Etch at voltages as low as 100 V for rapid and damage free preparation of sample surfaces.
- Permit samples as large as 32 mm in diameter.
- Transfer samples from the PECS™ II instrument to a SEM/FIB or glovebox without exposure to air (optional).
- Store and analyze image in DigitalMicrograph® software from Gatan for digital optical imaging.
- Display and control all PECS II parameters using integrated 10-inch color touch screen

Quorum Q150T-ES Sputter Coater

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Technical Specification:

The Q150T Plus is optimised for use with a turbomolecular pump, which gives a lower vacuum down to 5 x 10-5 mbar. This enables the sputtering of oxidising metals, which have a lower grain size suitable for high-resolution imaging. Similarly, lower scattering allows for high purity, amorphous carbon films of high density.

- New touch and swipe capacitive screen
- USB port for upgrades and download of process log files
- Multiple-user profiles can be set up on one machine
- New software sorts recipes per user according to recent use
- 16GB of memory can store more than 1000 recipes
- New multi-colour LED visual status indicator
- Capable of achieving vacuum of 5 x 10-5 mbar

Leica EM CPD300 Critical Point Dryer

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  • Technical Specification:

The drying of biological specimens such as pollen, tissue, plants, insects, as well as industrial samples, for example MEMS (Micro Electro Mechanical Systems) for SEM analysis can be prepared in the Leica EM CPD300 Critical Point Dryer, fully automatically. To ensure a low CO2 consumption and a very short process time a new filler concept was developed. Special attention has been turned to your safety by implementing software controlled cut-off functions and integrating a waste separator.

- High Sample Quality Fully automated, reproducible and controlled processes ensure high sample quality every run.
- Sample Holders Wide variety of sample holders for all of your sample sizes.
- Safe and easy disposal of exchange fluid Integrated liquid waste separator ensures safe and easy disposal of exchange fluid avoiding direct contract with user.

TEM Preparation Equipment:

Ultra microtomes

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  • Technical Specification:

Provides preparation of semi- and ultrathin sections in the range from 0,05 to 2,5 micrometres.

- Leica EM FC7 Cryochamber, ultrathin cryo-sectioning of hydrated samples at temperatures between -15°C to -185°C
- Reichert Ultracut S
- Reichert Ultracut FC S
- Glass knives are provided by the facility
- Diamond knives are provided by users

High Pressure Freezer (HPF): Leica EM HPM 100

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  • Technical Specification:

- The Leica EM HPM100 is a high pressure freezing system for vitrifying samples up to 200µm in thickness.
- The unique 6 mm diameter carrier system of the Leica EM HPM100 allows even more sample area to be frozen, like no other high pressure freezing instrument.
- The state-of-the-art design of the Leica EM HPM100 enables express sample handling and easy use with excellent freezing results.

Vitrobot Plunge Freezing

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  • Technical Specification:

Vitrobot automates the vitrification process to provide fast, easy, reproducible sample preparation - the first step in obtaining cryo-EM images.

- User-defined settings ensure maximum flexibility
- Automated and reproducible vitrification of suspensions on grids
- Controlled humidity and temperature environment
- Plunge freezing in liquid ethane or ethane-propane mixture

PELCO easiGlowTM

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  • Technical Specification:

For glow discharge applications, making TEM support films or grids hydrophilic using air, the PELCO easiGlow™ uses an automated and quick cycle with fully selectable parameters. The microprocessor controlled system also offers full manual control for all parameters and an advanced protocol programming feature for custom designed glow discharge applications. Parameters and protocols can be stored.

- Optimized for cleaning TEM grids
- Auto, programmed and manual mode
- Supports hydrophilic/hydrophobic and negative/positive modes

PELCO BioWave® Pro+

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  • Technical Specification:

PELCO BioWave® Pro+ enhances the processing for resin embedding for EM samples. Tissue and cell processing for TEM, FIB, immunofluorescence and correlative imaging takes approximately one day.

Low Vacuum Coater Leica EM ACE200

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  • Technical Specification:

Leica EM ACE200 produces homogenous and conductive metal or carbon coatings for SEM and TEM analyses.

Configured as a carbon thread evaporation coater, perfectly reproducible results can be achieved in the automated system.

Quartz Crystal Measurement controls carbon layer thickness.

Implen NanoPhotometer N60

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  • Technical Specification:

Microvolume spectroscopy with the convenience of a lightweight portable instrument with a built-in 7 inch LCD glove compatible touchscreen with flexible unit control options. Built-in vortex provides sample uniformity of homogenous samples for the highest degree of accuracy with the smallest sample volume with as little as 0.3 µl. Illuminated quartz sample surface allows for easy sample application.

Glass Knifemaker Leica EM KMR3

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  • Technical Specification:

The balanced break method of the Leica EM KMR3 ensures perfect glass knives in three thicknesses 6,4 mm, 8 mm and 10 mm.

The Leica EM KMR3 is easy to use and the automatic reset of breaking wheel and the scoring mechanism to "default" after a breaking cycle avoids handling errors.

Leica EM AFS2

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  • Technical Specification:

The Leica EM AFS2 performs freeze substitution and progressive lowering of temperature techniques, allowing low temperature embedding and polymerization of resins.

The Leica EM FSP (freeze substitution processor), an automatic reagent handling system combined with the Leica EM AFS2, dispenses reagents for freeze substitution. The LED illumination from within the chamber and the attached stereomicroscope for viewing and positioning of samples ensure ease of use.

Latest update: 2024-03-06