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The thylakoid lumen, a dynamic part of the photosynthetic process

Research project The beautiful architecture of the thylakoid network is a distinguishing characteristic of the chloroplast of higher plants. Mature thylakoids are differentiated into cylindrical grana stacks and tubular stroma lamellae that together enclose the continuous luminal compartment.

It is well established that the chloroplast lumen plays an important role in the formation of the thylakoid proton gradient that drives ATP synthesis. Almost nine years ago my group started a systematic study of the luminal content of the thylakoid membrane and was first with the discovery that the chloroplast lumen contained a large number of proteins. This study showed that the luminal chloroplast compartment contained an own specific proteome with several novel protein families.

Head of project

Project overview

Project period:

2008-04-23 2018-12-31

Funding

Finansår , 2003, 2004, 2005, 2006, 2007, 2008, 2009, 2010, 2011

huvudman: Wolfgang Schröder , finansiär: EU, y2003: , y2004: 600, y2005: 600, y2006: 300, y2007: , y2008: , y2009: , y2010: , y2011: ,

huvudman: Wolfgang Schröder, finansiär: Vetenskapsrådet, y2003: 350, y2004: 350, y2005: , y2006: , y2007: , y2008: , y2009: , y2010: , y2011: ,

huvudman: Wolfgang Schröder , finansiär: Carl Tryggers stiftelse, y2003: 400, y2004: , y2005: , y2006: , y2007: , y2008: , y2009: , y2010: , y2011: ,

huvudman: Wolfgang Schröder , finansiär: FORMAS, y2003: , y2004: , y2005: 473, y2006: 473, y2007: 473, y2008: , y2009: , y2010: , y2011: ,

huvudman: Thomas Kieselbach, Wolfgang Schröder, Gunnar Wingsle, Karin Ljung, finansiär: VR, KFI, y2003: , y2004: , y2005: , y2006: , y2007: , y2008: 3400, y2009: , y2010: , y2011: ,

huvudman: Jan Dekker, Wolfgang Schröder m fl, finansiär: MCRTN EU INTRO2, y2003: , y2004: , y2005: , y2006: 200, y2007: 200, y2008: 200, y2009: 200, y2010: 200, y2011: ,

huvudman: Jenny Reanult, Wolfgang Schröder, Thomas Kieselbach m fl, finansiär: COST FA0603, y2003: , y2004: , y2005: , y2006: , y2007: 50, y2008: 50, y2009: 50, y2010: 50, y2011: 50,

Participating departments and units at Umeå University

Department of Chemistry, Faculty of Science and Technology

Research area

Chemical sciences

Project description

We performed genome wide bioinformatics prediction of luminal chloroplast proteins, and based on this work we were able to predict that the luminal chloroplast proteome of Arabidopsis consists of 80 proteins. Although, the study of the expression of the luminal chloroplast proteins has clearly changed the model of the chloroplast lumen, the functions of most of the novel luminal proteins are not yet known.

The aim of this proposal is to further develop these studies to gain a more profound understanding of the functions of the luminal proteome.
Specific tasks are:
1.Biotic and abiotic stress effects on the lumen content
2. Analysis of function and structure of some specific luminal proteins (TL29, PrxQ)
3. Protein expression in specific photosynthetic mutants
The tasks will be addressed by use of 2D-difference gel electrophoresis (DIGE), the use of hydroponically grown plants for full nutrition control, and screening for the gene of interest in random T-DNA insertion databases.