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Research group of Lars-Anders Carlson

Structure and mechanism of viral replication complexes

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Staff photo Lars-Anders Carlson Lars-Anders Carlson Associate senior lecturer (assistant professor), other position

For more information about the lab, including open positions, visit our website here.

We study how viruses reshape the membranes of the cells they infect. Specifically, we study virus-induced organelles called replication complexes which are formed by positive-sense RNA viruses. This vast group of viruses cause human diseases ranging from common cold to hepatitis C and Zika. While positive-sense RNA viruses differ from one another in many respects, they all carry out drastic rearrangements of host-cell membranes, forming membrane-bound replication complexes which serve to produce new copies of the virus genome.

Our lab was recently established at Umeå University and we are looking for ambitious and enthusiastic people to join us at all levels. For details on currently open positions, go to the lab website.

Two orthogonal methods form the basis of our experimental work on replication complexes

Cryo-electron tomography of cells. This method is uniquely capable of visualising the macromolecular architecture of the interior of cells at (sub)nanometre resolution, thus enabling in situ structural biology. These studies are conducted at the world class instruments of the Umeå Core Facility Electron Microscopy (UCEM).

In vitro reconstitution using giant unilamellar vesicles (GUVs). This is a synthetic biology approach aimed at recreating membrane-localised biological processes from their individual components. Purified proteins, RNA, and synthetic model membranes (GUVs) are labelled with fluorophores, and their interactions are analysed by quantitative fluorescence microscopy conducted at the Biochemical Imaging Centre (BiCU).

Head of project

Staff photo Lars-Anders Carlson Lars-Anders Carlson Associate senior lecturer (assistant professor), other position