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Öhlund Lab

Pancreatic cancer research laboratory

Research overview

Pancreatic cancer is resistant to available cancer therapies, which is reflected in a low 5-year survival rate of below 8 %.This indicate that novel strategies to tackle the disease are needed.
The pancreatic tumor is characterized by a pronounced tumor stroma that surrounds the cancer cells. This tumor stroma contains nerves, vasculature, immune cells, extracellular matrix proteins and cancer-associated fibroblasts, that all interacts with the cancer cells and provide the cancer cells with important signals that regulate cancer cell growth and survival, and contribute to therapy resistance.

The overall goal for the lab is to get a deeper understanding in the stromal heterogeneity, and to reveal and explore potential drugable targets hidden within the stroma.
We aim to determine which factors in the stroma that are important in regulating cancer cell growth, survival, immune escape, and drug resistance by using genetically engineered mouse models of pancreatic cancer and advanced 3-dimentional cell culture systems.
Furthermore, we are developing and testing drugs that inhibit the most important stromal interactions with the aim to discover new methods to treat the disease.

People and projects

Daniel Öhlund (PI) 
M.D., Ph.D., Wallenberg Molecular Medicine Fellow

Daniel has over 10 years of experience in pancreatic cancer research, and has a specific interest in the pancreatic microenvironment. He obtained his Ph.D. at Umeå University 2010, and 2012-2016 he worked as a post-doctoral fellow in David Tuveson's lab at Cold Spring Harbor Laboratory. The research has been focusing on finding diagnostic and prognostic biomarkers for pancreatic cancer derived from the stroma, and lately also to understand the role of the stroma in disease development. The overall aim in is to find methods to be able to diagnose the disease earlier and to develop new treatment strategies by attacking the microenvironment. Since 2017 he is group leader at the Wallenberg Centre for Molecular Medicine at Umeå University and a resident physician at the Cancer Centre at Umeå University Hospital.

Mitesh Dongre
Senior Research Engineer / Lab manager

Mitesh has experience in studying bacterial quorum sensing dependent pathogenesis in gastro-intestinal pathogens. Mitesh obtained his PhD in India where he did proteomic studies to understand molecular mechanisms of pathogenesis in Vibrio cholerae. During recent years his research focussed mainly on identification and characterisation of new toxin-secretion pathways in bacteria. In the Öhlund lab his main task is to understand significance of human microbiome, especially the gut microbiome, in the etiology of pancreatic cancer. Additionally, he is also analysing pathophysiological effects of secreted bacterial membrane vesicles for pancreatic cancers.
At the lab Mitesh is also operating as lab manager and is responsible for the organoid culture laboratory.

Charlotte Nordström
Lab technician / Animal colony manager

Charlotte is a laboratory technician and has worked at the Oncology Research Laboratory since 1997. In the Öhlund lab she is managing the animal colony and has developed the routines for the animal house. She is also assisting in various animal experiments. Furthermore, she are experienced in cell culturing, immunohistochemical staining, In Situ Hybridization (using the RNA scope technology), and different animal surgery procedures.

Cedric Patthey
Senior Research engineer

Cedric has his background in developmental biology. In the past he has studied cell fate specification, maturation, and cell type evolution with a focus on the neurons. He got increasingly interested in the use of bioinformatic tools, and he has optimized techniques for transcription profiling of cell types and is now delving into single cell transcriptomics.
In the Öhlund lab Cedric is focusing on the characterization of the cell type diversity of cells present in the stroma of pancreatic cancer. The aim is to decipher the molecular mechanisms involved in the interactions between cancer cells and stroma, with a focus on fibroblasts and the neurons innervating the pancreas.

James Mason
Post-doctoral fellow

James is a post-doctoral researcher from the UK. For his Ph.D., he studied human pluripotent stem cell heterogeneity using Raman Spectroscopy and cell population modelling techniques at the University of Sheffield. He began researching pancreatic cancer at the Wallenberg Centre for Molecular Medicine in Umeå in 2017. Presently James' primary projects involve conducting high throughput screens of small molecules to identify therapeutics in pancreatic cancer using in vitro organoid culture models as well as developing Raman spectroscopy to swiftly profile the disease in patients. Additionally, James studies tumour organoid metastatic potential, their susceptibility to ClyA toxin and quadruplex DNA dynamics.

Kumail Kumar Motiani
Post-doctoral fellow

Kumail completed his medical studies in Pakistan and started his PhD at the University of Turku. He has experience in studying internal organ metabolism using different radiological imaging modalities (PET, CT, MRI). In the Öhlund lab he will be responsible for developing radiotracers for early detection of pancreatic cancer. Additionally, he will be developing guidelines using different imaging modalities (CT, ultrasound) which will aid in diagnosis of both pancreatitis and pancreatic cancer. Kumail will also focus on radiotracers targeting the tumor stroma.

Paromita Kundu
Post-doctoral fellow

Paromita obtained her PhD in Biotechnology at the Institute of Life Sciences, India in 2018, where her research topic was “Nanotechnology based drug delivery system for neurodegenerative disorders”. Her research aimed to develop nanotechnological devices for delivery of neurotherapeutics to the brain where she formulated lipid based nanoparticles for delivering dual drug and studied anti-parkinsonism effect of both drugs in a Parkinson Disease mouse model.
In September 2019, she joined the Öhlund lab and her current research is focused on Nerve tumour interaction and the role of Schwann cells in initiation and progression of pancreatic cancer.

Ankit Kumar Patel
Post-doctoral fellow

Ankit has previously, during his Ph.D.-studies, established primary culture cell lines of cancer associated fibroblasts (CAFs) from gingivobuccal oral cancer patients. He has studied their phenotypic and functional characteristics and reported that CAFs are heterogeneous stromal cell types with distinct gene expression profile. These heterogeneous CAFs interacts differently with oral cancer cells and significantly affect the tumor biology. In Daniel Öhlund’s laboratory, he is studying the interaction of pancreatic CAFs in a co-culture system with pancreatic cancer organoids. He is studying the effect of chemotherapeutic drugs on the CAF-tumor organoid model system to delineate the druggable molecular pathways for better therapy.

Tommy Lidström 
Ph.D.-student

Tommy is a former student of the Biomedicine program at Umeå University, he previously researched the effect of serotonin receptor 1A in B-cell lymphoma, before joining group Daniel Öhlund as a Ph.D. student. Tommy's current research involves characterizing interactions between the extracellular matrix (ECM) and cancer cells in pancreatic ductal adenocarcinoma (PDAC). Currently Tommy is involved in two projects, the first is investigating how different types of cancer associated fibroblasts (CAFs) are formed via signaling-interactions with cancer cells, the second involves investigating the function and biological relevance of up- and down-regulated ECM proteins in PDAC.

Joshua Cumming
Ph.D.-student

Joshua completed his Bachelor's and Master's degrees at the University of Manchester before working as a research assistant at the Manchester Collaborative Centre for Inflammation Research (MCCIR). His previous research used super-resolution microscopy to assess the nanometer-scale organization of inhibitory receptors in natural killer cells. Joshua's PhD project will focus on identifying and characterizing subpopulations of cancer associated fibroblasts (CAFs) in pancreatic cancer. Joshua will be investigating whether manipulating CAF subtype differentiation is a viable therapeutic strategy for pancreatic cancer.

Other students:

Ismael Cem Nass Kebapcioglu
Master student
(April 2019-

Linda Ogol
Master student
(February 2020-

Tony Ullman
Bachelor student
(April 2020-

Former lab members:

Ismail Chergui
IFMSA student
(August 2019)

Silvia Remeseiro
Post-doctoral fellow
(April 2017-March 2019)

Sushmitha Sankarasubramanian
Master student
(April 2018 - January 2019)

Marcus Fredriksson Sundbom
Med. student, summer stipend
(June 2018 - August 2018)

Lucas de Barros Anastácio
IFMSA student
(June 2018)

Sofía Alejandra Moreira Lino
IFMSA student
(June 2018)

Rituparna De
Post-doctoral fellow
(April 2017-March 2018)

Fredrik Trulsson
Research project assistent
(September 2017 - December 2017)

Open positions in the lab

No positions are currently available. For questions regarding future positions, please contact Daniel (daniel.ohlund@umu.se). For questions regarding master thesis projects, please contact Mitesh (mitesh.dongre@umu.se). 

Find the lab

We are located in the 6M building at the Umeå University hospital campus.
Entrance L31 (close to the Medical Library)

Support our research

Donations can be sent to the lab (through Cancerforskningsfonden i Norrland).

Bg: 684-4468
Pg: 68 44 46-8
(Mark the transaction: Öhlund)

Forskningsledare

Daniel Öhlund
Biträdande universitetslektor, ST-läkare, övrig/annan befattning
E-post
E-post
Telefon
090-785 17 27

Publikationer

2020
Cancer Research, The American Association for Cancer Research 2020, Vol. 80, (7) : 1461-1474
Tian, Chenxi; Öhlund, Daniel; Rickelt, Steffen; et al.
2020
Journal of the American Chemical Society, Vol. 142, (6) : 2876-2888
Jamroskovic, Jan; Doimo, Mara; Chand, Karam; et al.
2019
Clinical Cancer Research, Vol. 25 : 6742-6755
Ponz-Sarvise, Mariano; Corbo, Vincenzo; Tiriac, Hervé; et al.
2019
Journal of Pathology, John Wiley & Sons 2019, Vol. 5, (2) : 130-141
Franklin, Oskar; Billing, Ola; Öhlund, Daniel; et al.
2019
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences 2019, Vol. 116, (39) : 19609-19618
Tian, Chenxi; Clauser, Karl R.; Öhlund, Daniel; et al.
2019
Gut, BMJ Publishing Group Ltd 2019, Vol. 68, (1) : 159-171
Neesse, Albrecht; Bauer, Christian Alexander; Öhlund, Daniel; et al.
2018
Annals of Surgery, Vol. 267, (4) : 775-781
Franklin, Oskar; Jonsson, Pär; Billing, Ola; et al.
2018
Journal of Pathology, John Wiley & Sons 2018, Vol. 246, (3) : 311-322
Morin, Eric; Sjöberg, Elin; Tjomsland, Vegard; et al.