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Umeå universitet
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Huvudmenyn dold.
Personalbild Paulina Wanrooij

Paulina Wanrooij

Min forskargrupp studerar mitokondriellt DNA och hur dess instabilitet signaleras till resten av cellen.

Vetenskaplig meritering: Docent
0000-0002-8607-7564

Kontakt

E-post
paulina.wanrooij@umu.se
Telefon

Verksam som

Anknytning
Forskare vid Institutionen för medicinsk kemi och biofysik
Plats
KB. H6, Campus, Umeå Umeå universitet, 901 87 Umeå

Främsta syftet med vårt arbete är att förstå mekanismer som antingen förhindrar eller hjälper till att hantera olika former av mtDNA-instabilitet. Våra forskningsprojekt rör allt från vad som krävs för felfri kopiering av mtDNA:t till hur mtDNA defekter kommuniceras till resten av cellen och vilka konsekvenser det får. Vår forskning kommer att leda till ökad förståelse för orsakerna och konsekvenserna av mtDNA instabilitet, vilket kan hjälpa oss identifiera potentiella mål för framtida läkemedelsutveckling.

MRE11-independent effects of Mirin on mitochondrial DNA integrity and cellular immune responses
Molecular Biology of the Cell, American Society for Cell Biology (ASCB) 2025, Vol. 36, (2)
Aasumets, Koit; Hangas, Anu; Fragkoulis, Georgios; et al.
RRM2B deficiency causes dATP and dGTP depletion through enhanced degradation and slower synthesis
Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences (PNAS) 2025, Vol. 122, (16)
Awoyomi, Ololade Folajimi; Gorospe, Choco Michael; Das, Biswajit; et al.
The low endoribonuclease activity and lack of rNMP preference of human mitochondrial topoisomerase 1 protect against ribonucleotide-dependent deletions
Nucleic Acids Research, Oxford University Press 2025, Vol. 53, (11)
Bader, Cyrielle P.J.; Miyazaki-Kasho, Erika; Forslund, Josefin M.E.; et al.
Activating AMPK improves pathological phenotypes due to mtDNA depletion
The FEBS Journal, John Wiley & Sons 2025, Vol. 292, (9) : 2359-2380
Carvalho, Gustavo; Nguyen, Tran V. H.; Repolês, Bruno Marçal; et al.
The POLγ Y951N patient mutation disrupts the switch between DNA synthesis and proofreading, triggering mitochondrial DNA instability
Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences (PNAS) 2025, Vol. 122, (16)
Forslund, Josefin M.E.; Nguyen, Tran V. H.; Parkash, Vimal; et al.
PrgE: an OB-fold protein from plasmid pCF10 with striking differences to prototypical bacterial SSBs
Life Science Alliance, Life Science Alliance 2024, Vol. 7, (8)
Breidenstein, Annika; Lamy, Anaïs; Bader P.J., Cyrielle; et al.
Mitochondrial membrane potential acts as a retrograde signal to regulate cell cycle progression
Life Science Alliance, Life Science Alliance, LLC 2023, Vol. 6, (12)
Gorospe, Choco Michael; Carvalho, Gustavo; Herrera Curbelo, Alicia; et al.
Determination of the ribonucleotide content of mtDNA using alkaline gels
Mitochondrial DNA: methods and protocols, New York: Humana Press 2023 : 293-314
Gorospe, Choco Michael; Repolês, Bruno Marçal; Wanrooij, Paulina H.
NME6: ribonucleotide salvage sustains mitochondrial transcription
EMBO Journal, John Wiley & Sons 2023, Vol. 42, (18)
Wanrooij, Paulina H.; Chabes, Andrei
Mitochondrial DNA Instability in Mammalian Cells
Antioxidants and Redox Signaling, Mary Ann Liebert 2022, Vol. 36, (13-15) : 885-905
Carvalho, Gustavo; Repolês, Bruno Marçal; Mendes, Isabela; et al.
The integrity and assay performance of tissue mitochondrial DNA is considerably affected by choice of isolation method
Mitochondrion (Amsterdam. Print), Elsevier 2021, Vol. 61 : 179-187
Repolês, Bruno Marçal; Gorospe, Choco Michael; Tran, Phong; et al.
MtDNA replication, maintenance, and nucleoid organization
The human mitochondrial genome: from basic biology to disease, Academic Press 2020 : 3-33
Doimo, Mara; Pfeiffer, Annika; Wanrooij, Paulina H.; et al.
Elimination of rNMPs from mitochondrial DNA has no effect on its stability
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences 2020, Vol. 117, (25) : 14306-14313
Wanrooij, Paulina H.; Tran, Phong; Thompson, Liam J.; et al.
De novo dNTP production is essential for normal postnatal murine heart development
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology 2019, Vol. 394, (44) : 15889-15897
Tran, Phong; Wanrooij, Paulina H.; Lorenzon, Paolo; et al.
Ribonucleotides in mitochondrial DNA
FEBS Letters, John Wiley & Sons 2019, Vol. 593, (13) : 1554-1565
Wanrooij, Paulina H.; Chabes, Andrei
The presence of rNTPs decreases the speed of mitochondrial DNA replication
PLOS Genetics, Public library science 2018, Vol. 14, (3)
Forslund, Josefin M. E.; Pfeiffer, Annika; Stojkovič, Gorazd; et al.
Inosine Triphosphate Pyrophosphatase Dephosphorylates Ribavirin Triphosphate and Reduced Enzymatic Activity Potentiates Mutagenesis in Hepatitis C Virus
Journal of Virology, American Society for Microbiology 2018, Vol. 92, (19)
Nystrom, Kristina; Wanrooij, Paulina H.; Waldenstrom, Jesper; et al.
DNA Damage Tolerance by Eukaryotic DNA Polymerase and Primase PrimPol
International Journal of Molecular Sciences, MDPI AG 2017, Vol. 18, (7)
Boldinova, Elizaveta O.; Wanrooij, Paulina H.; Shilkin, Evgeniy S.; et al.
Inosine triphosphate pyrophosphatase enhances the effect of ribavirin on hepatitis C virus cell culture infection
Journal of Hepatology, ELSEVIER SCIENCE BV 2017, Vol. 66, (1) : S321-S321
Nystrom, K.; Pettersson, G.; Wanrooij, Paulina; et al.
Ribonucleotides incorporated by the yeast mitochondrial DNA polymerase are not repaired
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences 2017, Vol. 114, (47) : 12466-12471
Wanrooij, Paulina H.; Engqvist, Martin K. M.; Forslund, Josefin M. E.; et al.
The dimeric architecture of checkpoint kinases Mec1(ATR) and Tel1(ATM) reveal a common structural organization
Journal of Biological Chemistry, Elsevier 2016, Vol. 291, (26) : 13436-13447
Sawicka, Marta; Wanrooij, Paulina H.; Darbari, Vidya C.; et al.
Oxidative DNA damage stalls the human mitochondrial replisome
Scientific Reports, Vol. 6
Stojkovic, Gorazd; Makarova, Alena V.; Wanrooij, Paulina H.; et al.
Probing the Mec1ATR Checkpoint Activation Mechanism with Small Peptides
Journal of Biological Chemistry, Vol. 291, (1) : 393-401
Wanrooij, Paulina H.; Tannous, Elias; Kumar, Sandeep; et al.
Cancer-derived mutations in the mitochondrial single-stranded DNA-binding protein impair DNA binding and thermal stability
Bader, Cyrielle P.J.; Dash, Aiswarya; Forslund, Josefin M.E.; et al.
Visa publikationer i DiVA

Forskargrupper

Forskningsledare
Paulina Wanrooij lab

Forskningsprojekt

1 januari 2020 till 31 december 2022
Checkpoint-mekanismer som skyddar vårt mitokondriella genom
Film: Hur behålls mitokondriella DNA i felfri form?
Hur behålls mitokondriella DNA i felfri form?

Paulina Wanrooij är intresserad av genmaterialet i organellerna, mitokondrier, som producerar merparten av cellens energi.